Mature adult male hamsters are very easy to differentiate by observing a large buttocks at the rear.
However, young hamsters are usually differentiate by inverting them around and check their private areas in between their hind legs.
Generally, baby/infant female pups have a shorter distance between the 2 holes spotted at their rear while baby/infant male pups have a longer distance between the 2 holes spotted at their rear.
This is usually done through experienced eyes as non-experienced people will have problem differentiate.
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